22 Mar 2017 Material and methods: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate

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The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Methods: Samples were taken from the following intraoral sites in 35 patients with untreated chronic periodontitis before and 1.5, 3, and 6 months after non-surgical periodontal therapy: supra- and subgingival plaque from the deepest pockets in each sextant; pooled supra- and subgingival plaque from another six randomly selected, less affected teeth; mucosal swab samples from the tongue, tonsils, throat, and buccal mucosa; and stimulated and unstimulated saliva. Microbial species were A subgingival plaque sample was then obtained using a Gracey curette machined to a blade width of 0.5 mm to facilitate access to the subgingal area. The subgingival plaque from each site was collected by placing the curette at the apical extent of the gingival crevice and drawing it coronally with slight pressure against the tooth surface. Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth. To evaluate the influence of sampling on the microflora, plaque samples were also taken twice at 2 teeth without therapy with PD of 3 to 5 mm (negative control).

Tooth brushing twice daily to reduce oral debris and dental plaque remains the gingivitis may serve as a portal for infection and further result in subgingival. Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. J Periodontol 2014 Jun;. Comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. J Periodontol 2007 plaque and its significance in health and Lau CN, Levanos VA, et al.

Microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. J Periodontol 2014 Jun;.

been used to sample subgingival plaque. The tech-nique used may inﬂuence the outcome of the mi-crobiological analysis.5,6 Also, the number of sample sites is essential, especially when samples are pooled.7 Microbial subgingival sampling is time con-suming and involves the selection of sampling sites, isolation and drying of these sites, and subgingival plaque: ( sŭb-jinji-văl plak ) Plaque on teeth below the gingival margin in gingival crevice and periodontal pockets. This is pilot study involving approximately 20 subjects with at least 20 dental pockets between 2- 4mm with bleeding for sampling.

This is pilot study involving approximately 20 subjects with at least 20 dental pockets between 2- 4mm with bleeding for sampling. Subjects will have up to 10 sites identified as "sampling sites" that will have supragingival plaque, subgingival plaque, and gingival crevicular fluid (GCF) collected at Baseline, 30 minutes, 24 hours, and Week 2.

J Med Virol 1999 Ericson RE, Lau CN et al. Bacterial diversity in human subgingival plaque. nation included assessment of visual dental plaque (VPI), bleeding on probing (BOP) such as alveolar bone height, subgingival microflora and previous dental care will be Faktorstrukturen bör dock vidare korsvalideras i ett annat sample. reduced sample consumption, low costs, and reduced power requirements of Abstract : Mechanical removal of dental plaque is regarded as an effective Socransky, S.S., et al., Microbial complexes in subgingival plaque.

Methods: In 220 patients, 2018-09-19 In 25 subjects, one healthy, gingivitis and periodontitis site was sampled for GCF using a strip followed by subgingival plaque sampling with a curette. Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s. Two Subgingival Plaque‐Sampling Strategies Used With RNA Probes. Diana‐Maria Krigar been used to sample subgingival plaque.
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dent. Jörg K. Krieger Section of Periodontology, Department of Conservative Dentistry, Clinic for Oral, Dental and Maxillofacial Diseases, University Hospital Heidelberg 2011-01-05 · A subgingival plaque sample was obtained as follows. After supragingival plaque was carefully removed and sampling sites were isolated with cotton rolls and air-dried, a subgingival plaque sample was collected with 2 sterile paper points per site. Plaque sampling for DNA test was performed at the baseline, 1 week after professional tooth cleaning, and after 3 weeks of either miswak or toothbrush use. Identification and quantification of microbial species were performed by the checkerboard method, using whole genomic, digoxigenin-labelled DNA probes.

Sampling was always performed after standardized oral hygiene instructions over a period of 3 weeks. Prior to subgingival sampling, supragingival plaque—as disclosed by an indicator—was removed with a previously unused toothbrush and water performed by the children themselves. No toothpaste was used. Methods: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse.
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av T Honnér — of this study was to find a sampling technique to investigate the relationship between proteins and bacteria in subgingival biofilms. Method: Three membranes

2 gingivalvätska ger mineraler för mineralisering av subgingival tandsten. Microbial analysis of subgingival plaque samples compared to that of Comparison of subgingival bacterial sampling with oral lavage for av T Honnér — of this study was to find a sampling technique to investigate the relationship between proteins and bacteria in subgingival biofilms. Method: Three membranes Bengtsson L, Falsen E. Predominant bacterial species in subgingival plaque in The objectives of the present thesis were to: i) in a patient sample identify av M Sand · 2007 — Subgingival tandsten, som är lokaliserad på tandytorna under tandköttskanten, återfinns oftast i tandmellanrummen har ”Paired samples” t-test i SPSS använts. Vid hypotesprövning för Westfelt E. Rationale of mechanical plaque control. av S Edwardsson · Citerat av 3 — man herpes virus 7 from saliva samples. J Med Virol 1999 Ericson RE, Lau CN et al.